I am a PhD student working in Cans Group. My focus is to develop enzyme-based ultrafast electrochemical biosensors to monitor the rapid exocytosis events that release non-electroactive neurotransmitters like glutamate and acetylcholine on millisecond timescale. I also work on the interaction between nanoparticles and enzyme in bulk solution to study the effect molecular crowding on enzymatic activity. Besides, we try to establish novel methods using nano-sized artificial liposome to quantify the non-electroactive neurotransmitters in single synaptic vesicles. I came to Sweden for a master degree of Biotechnology in tissue engineering at Chalmers University of Technology from 2011 with a  bachelor degree of Bioengineering.

 

 

  • Molecular Crowding and a Minimal Footprint at a Gold Nanoparticle Support Stabilize Glucose Oxidase and Boost its Activity. Wang, Y, Jonkutė, R, Lindmark, H, Keighron, J. D, Cans, A.-S.* 2019, Under Review
  • Counting the Number of Glutamate Molecules in Single Synaptic Vesicles. Wang, Y, Fathali, H, Mishra, D, Olsson, T, Keighron, J. D, Skibicka, K. P, Cans, A.-S. J. Am. Chem. Soc. 2019, 141 (44), 17507–17511.  DOI: https://doi.org/10.1021/jacs.9b09414
  • Creating Ultrafast Biosensors for Neuroscience, Wang, Y. Doctoral Thesis, 2019. ISBN:
    978-91-7905-150-1
  • Ultrafast Glutamate Biosensor Recordings in Brain Slices Reveal Complex Single Exocytosis Transients. Wang Y, Mishra D, Bergman J, Keighron JD, Skibicka KP, Cans A-S.ACS Chem. Neurosci. DOI:10.1021/acschemneuro.8b00624.
  • Co-Detection of Dopamine and Glucose with High Temporal Resolution. Bergman, J. Mellander, L., Wang, Y., Cans, A-S.* Catalysts. 2018;8(1). doi:10.3390/catal8010034.
  • Counting the Number of Enzymes Immobilized onto a Nanoparticle Coated Electrode. Bergman J., Wang Y., Wigström J., and Cans A-S.* (2017) Analytical and Bioanalytical Chemistry.
  • Keighron, J. D., Wigström, J., Kurczy, M. E., Bergman, J., Wang, Y., & Cans, A.-S. (2015). Amperometric Detection of Single Vesicle Acetylcholine Release Events from an Artificial Cell. ACS Chemical Neuroscience, 6(1), 181–188.
  • The 17th biennial International Conference on Molecules in Neuroscience meeting, Oxford, UK, March, 2018
  • Advance Nanoscience and Nanotechnology 6th Annual Meeting, Falkenberg, Sweden, August, 2017
  • Biosensors 2016, Gothenburg, Sweden, May, 2016
  • The Pittsburgh Conference & Exposition 2016 (PITCCON 2016) at Atlanta, U.S.A, March, 2016
  • Biophysical Society (PBS) 60th Annual Meeting at Los Angeles, U.S.A. February, 2016
  • Poster for the 16th Beijing Conference and Exhibition on Instrumental Analysis, China, 2015 Oct 27-30
  • Poster for the International Symposium on In Vivo Analysis, Beijing, China, 2015 Oct 25-26
  • Advance Nanoscience and Nanotechnology 4th Annual Meeting, Brastad, Sweden, 2015 Aug 24-26
  • Nobel Workshop, Gothenburg, Sweden, 2015 May 4-8
  • Poster for the 59th Biophysical Society Conference, Baltimore, U.S., 2015 Feb 7-11
  • Advance Nanoscience and Nanotechnology 3rd Annual Meeting, Uddevalla, Sweden, 2014 Jun 16-18
  • Thesis Projects
  • -Bachelor Thesis Project, Main Supervisor
  • Optimization and design of enzyme based biosensors for high temporal recording of molecules important in brain chemistry, Hampus Lindmark, 2016, Gothenburg, Sweden
  • -Bachelor Thesis Project, Main Supervisor
  • Characterisation and study of enzyme-based electrochemical biosensors, Sanna Johansson, Ylva Johansson, 2017, Gothenburg, Sweden

 

  • Physical Chemistry
  • - Determination of Dissociation Energy of Iodine by Spectroscopy method
  • Chemistry and Biochemistry
    - Vegetables pH indicators
    - Theoretical molecular calculations: 3D geometry, energy, and molecular orbitals.
    - Basic operations in the laboratory: Extraction/Crystallization/Distillation
  • Advanced Analytical Chemistry
    -High Performance Liquid Chromatography_Determination of Caffeine and Benzoic Acid in Coffee
    -Spectrophotometry: Determination of Fluoride in Tooth Paste

 

EDUCATION
  • 2019-Now         Researcher, Analytical Chemistry, Department of Chemistry and Chemical Engineering, Chalmers University of Technology (CTH), Gothenburg, Sweden
  • 2014-2019     PhD student, Analytical Chemistry, Department of Chemistry and Chemical Engineering, CTH, Gothenburg, Sweden
  • 2012-2013       Master Thesis in Biomedical Photonics Group, University of Gothenburg, Sweden
  • 2011-2013    Master Degree in Biotechnology, CTH, Gothenburg, Sweden
RESEARCH HISTORY
  • 2013 Jun – Nov           Project Assistant, Innovitro AB, Sweden

The work in Innovitro AB was a further research of my master thesis project which intended to develop a novel in vitro method called HaptoGrade. HaptoGrade method which has been patented by Innovitro AB measures the blebbing response together with other morphologic changes of cells to chemicals to grade sensitizing potency of chemicals, instead of animals. I was responsible for performing two blind tests for a major chemical company and a major fragrance company, respectively. This job added me skills in cellular cultivation, imaging live cells using high content screening microscopy. I was more confident of my ability to plan my own experiments, analyzing a large amount of data and presenting the complex data to my colleagues.

  • 2013 Sept - Oct            Scientist, Medibiome AB, Sweden

During the 2 months of work in Medibiome AB as a scientist, I got practical experience of gram staining and cultivating human skin and microorganism. I documented about 30 common apparatus and 20 specious of microorganism in the laboratory, wrote study protocol with detail steps, time plan and budget for a tender, and communicated with our suppliers and customers when necessary.

  • 2012 Sept - 2013 Jun   Master thesis, Biomedical Photonics Group, University of Gothenburg, Sweden

I finished a 60 credits master thesis to further develop and optimize HaptoGrade method during this period. I independently planed and performed experiments involving cellular cultivation, live cell imaging using optical microscopy, analyzing 50,000 images and assisting IT technicians from Fraunhofers to develop the automated software. I also independently set up another immortalized cell line (i.e. HaCaT) which might be used as a substitute. Besides, I prepared several posters and made oral presentations at different international conferences (e.g. OEESC conference in Netherlands, 2013).

  • 2012 Jan - May              Research Assistant, Magle Life Sciences AB, Sweden

I co-cultured mouse fibroblasts and human endothelial cells with different starch microspheres for a half-year course project in CTH. Its aim is to develop a novel microsphere which can assist wound healing. The attachment of cells to microsphere was observed using fluorescent microscopy, and images were analyzed via Image J. This project added me knowledge in wound healing process and different inflammatory diseases (e.g. diabetes) as well.

  • Chalmers Research Fund, Chalmers University of Technology, 2015 & 2017
  • IPOET scholarship, Chalmers University of Technology, 2011-2013